Design and delivery of messenger RNA-based vaccines

Nonviral delivery of self-amplifying RNA vaccines.

Despite more than two decades of research and development on nucleic acid vaccines, there is still no commercial product for human use. Taking advantage of the recent innovations in systemic delivery of short interfering RNA (siRNA) using lipid nanoparticles (LNPs), we developed a self-amplifying RNA vaccine. Here we show that nonviral delivery of a 9-kb self-amplifying RNA encapsulated within ...

Development of Delivery Strategies Facilitating Broad Application of Messenger Rna Tumor Vaccine by Development of Delivery Strategies Facilitating Broad Application of Messenger Rna Tumor Vaccine By

Genetic modification of dendritic cells with plasmid DNA is plagued with low transfection efficiencies because DNA taken up by non-dividing dendritic cells rarely reaches the nucleus. But this difficulty can be overcome by the use of messenger RNA (mRNA), which exerts its biological function in the cytoplasm and obviates the need to enter the nucleus. Since pioneering work of Boczkwoski et al.,...

Messenger RNA.

Sublingual Delivery of Vaccines

Sublingual immunization, where the vaccine is placed directly under the tongue and absorbed through the sublingual mucosa, has the advantages of ease of delivery without the complication of oral administration or the safety concerns of intranasal vaccines. Sublingual vaccine delivery has been shown to induce development of humoral and cell mediated immunity in both the systemic and mucosal comp...

Messenger RNA Modulated by Thioacetamide Increased Activity of Rat Liver Messenger RNA and of Albumin

Administration of thioacetamide to rats was found to increase the activity of liver messenger RNA in the wheat germ protein synthesis assay. The synthesis of total protein was increased about 2.5-fold after 4 days of treatment. The treatment was associated with an increase in the relative quantity of polyadenylic acid-containing RNA. Immunoprecipitation studies showed that albumin synthesis dir...